Molekularno genetička osnova tumora gastrointestinalnog sustava

Course

Molekularno genetička osnova tumora gastrointestinalnog sustava

ECTS

3.5

Status

Optional course

Execution of classes

P16/S4

Course performers

dr. sc. Sanja Kapitanović, Voditelj predmeta
dr. sc. Tamara Čačev, Nastavnik
dr. sc. Marijana Popović- Hadžija, Nastavnik
izv. prof. dr. sc. Maja Sirotković Skerlev, Nastavnik
dr. sc. Maja Cigrovski Berković, Nastavnik

Studies on which it's performed

BIOMEDICINA I ZDRAVSTVO

Competencies and learning outcomes

Basic understanding of advances in the field of molecular medicine in gastroenterology and their possible application in every day clinical practice.

Contents

Introduction to the principles involved in cancer development and progression, based on the model of gastrointestinal tumors. Review of molecular changings in oncogenes, tumor supressor genes and mismatch repair genes relating to illneses of the gastrointestinal tract will be given. Course topics include the genetics of hereditary and sporadic colon cancer, familial adenomatous polyposis (FAP) and human nonpolyposis colon cancer (HNPCC, Lynch syndrome). The gastric carcinoma is second most freequent gastrointestinal tumor in patients with HNPCC and the lectures will include the genetics of hereditary and sporadic gastric cancer. Course topics will be also the most recent findings in: molecular genetics of other human polyposis syndromes (Syndroma Gardner, Peutz-Jeghers, juvenile polyposis); molecular genetics of inflammatory bowel disease (IBD) (ulcerative colitis, Crohn’s disease); molecular genetics of pancreatitis and pancreatic adenocarcinoma. Molecular genetics of GEP-NETs tumors. Chemoprevention is another approach to decrease the incidenece of colorectal cancer in the general population. One of the topics of this course will be the role of COX-2 in the tumorigenesis and as a new molecular target in chemoprevetion and treatment of gastrointestinal tumors. Nutrigenomics, nutrigenetics of gastrointestinal tumors. Pharmacogenomics and pharmacogenetics of gastrointestinal tumors, importance of SNPs and mutations in genes for drug metabolism, response to therapy, toxicity and resistence to therapy. Seminar topics will include: molecular diagnostics of hereditary gastrointestinal tumors, presymptomatic diagnostics, pharmacogenetics, interpretation of the results of molecular-genetics analysis (mutation detection and "linkage" analysis).

Literature

1. Kinzler KW i Vogelstein B. Lesons from hereditary colorectal cancer. Cell 87:159, 1996.
2. Chung DC. The genetic basis of colorectal cacer: insights into critical pathways of tumorigenesis. Gastroenterlogy 19:854, 2000.
3. Kartheuser A i sur. The genetic background of familial adenomatous polyposis. Linkage analysis, the APC gene identification and mutation screening. Acta Gastro-Enterol Belg 58:433, 1995.
4. Fodde R i sur. APC, signal transduction and genetic instability in colorectal cancer. Nature Rev Cancer 1:55, 2001.
5. Lynch HT i sur. Overview of natural history, pathology, molecular genetics and management of HNPCC (Lynch syndrome). Int J Cancer 69:38, 1996.
6. Ward R i sur. Impact of microsatellite testing and mismatch repair protein expression on the clinical interpretation of genetic testing in hereditary non-polyposis colorectal cancer. J Cancer Res Clin Oncol 128:403, 2002.
7. Rhodes JM i Campbell BJ. Inflammation and colorectal cancer: IBD-associated and sporadic cancer compared. Trends Mol Med 8:10, 2002.
8. Jankowski JA i sur. Molecular evolution of the metaplasia-dysplasia-adenocarcinoma sequence in the esophagus. Am J Pathol 154:965, 1999.
9. Caldas C i sur. Familial gastric cancer: overview and guidelines for management. J Med Genet 36:873, 1999.
10. Werner M i sur. Gastric adenocarcinoma: pathomorphology and molecular pathology. J Cancer Res Clin Oncol 127:207, 2001.
11. Gupta RA i DuBois RN. Colorectal cancer prevention and treatment by inhibition of cyclooxygenase-2. Nature Rev 1:11, 2001.
12. Rabelo R i sur. Role of molecular diagnostic testing in familial adenomatous polyposis and hereditary nonpolyosis colorectal cancer familes. Dis Colon Rectum 44:437, 2001.
13. Loktionov A. Common gene polymorphisms, cancer progression and prognosis. Cancer Letters 208:1-33, 2004.
14. Cancer Pharmacogenomics: SNPs, chips, and the individual patient. Cancer Invest 21:630-640, 2003.
15. Yamayoshi Y i sur. Cancer pharmacogenomics: international trends. Int J Clin Oncol 10:5-13, 2005.

Additional reading

Teaching forms

Closing seminar: critical analysis of a selected scientific paper related to the course content ("journal club").

Examination procedure

Written exam (test)