Projekt HRZZ: Novi biomarkeri kronične bolesti presatka protiv primatelja

Naziv projekta Hrvatske zaklade za znanost: Novi biomarkeri kronične bolesti presatka protiv primatelja

Ustanova: Sveučilište u Zagrebu Medicinski fakultet

Voditelj projekta: doc. dr. sc. Dražen Pulanić, dr. med.

Trajanje projekta: 48 mjeseci
Kronična bolest presatka protiv primatelja (eng. chronic Graft-versus-Host disease, cGVHD) je multiorganska aloimuna i autoimuna bolest koja je najvažnija kasna komplikacija nakon alogenične presadbe krvotvornih matičnih stanica (aloPKMS) i vodeći uzrok ne-relapsnog morbiditeta i smrtnosti nakon aloPKMS-a. Zapažena incidencija bolesti kreće se između 30-70% nakon aloPKMS-a i povećava se zbog sve manjeg peritransplantacijskog mortaliteta, starije dobi transplantiranih bolesnika, češće upotrebe perifernih krvotvornih matičnih stanica i sve većeg broja transplantacija od nesrodnog davatelja. Bolest je kompleksna i obično zahvaća više različitih organskih sustava (kožu, oči, usta, jetru, gastrointestinalni sustav, pluća, muskuloskeletni sustav i genitourinarni sistem), a često se prezentira manifestacijama koje nalikuju na promjene u autoimunosnih bolesti. Nužno je pronalaženje prediktivnih i dijagnostičkih biomarkera koji bi koristili u procjeni rizika nastanka, dijagnoze i praćenja, procjene ativnosti i ishoda kroničnog GVHD-a. U Kliničkom bolničkom centru Zagreb 2013. godine osnovan je multidisciplinarni tim stručnjaka za cGVHD po uzoru na onaj formiran na National Institutes of Health (NIH, Bethesda, SAD). Osim što doprinosi poboljšanju skrbi za oboljele od cGVHD-a, ovaj tim omogućuje preciznu interdisciplinarnu kliničku i laboratorijsku evaluaciju bolesti, prikupljanje bioloških uzoraka te nudi veliki potencijal za ispitivanje biomarkera ove teške i potencijalno smrtonosne kasne komplikacije nakon aloPKMS-a.
Cilj ovog novog četverogodišnjeg projekta Hrvatske zaklade za znanost je prospektivnim longitudinalnim istraživanjem ispitati moguće nove biomarkere kroničnog GVHD-a (analizu glikana, faktora koagulacije, laboratorijskih parametara upale, subpopulacije limfocita i monocita) uz detaljno kliničko multidisciplinarno evaluiranje bolesnika korištenjem suvremenih NIH standarda za cGVHD.

 

Croatian Science Foundation Project title: New biomarkers for chronic graft-versus-host disease

Institution: University of Zagreb School of Medicine

Project leader: Assist. Prof. Dražen Pulanić, MD, PhD

Project duration: 48 months

Chronic graft-versus-host disease (cGVHD) is a multi-organ alloimmune and autoimmune disorder and the most important late complication after allogeneic hematopoietic stem cell transplantation (alloHSCT). It is the major cause of non-relapse morbidity and mortality after alloHSCT. Reported incidence rates of cGVHD range from 30-70% of patients after alloHSCT, and are increasing due to lower peritransplant mortality, older recipients, the more frequent use of peripheral blood graft, and more unrelated donors. It is a complex and multisystem disease, affecting skin, eyes, mouth, liver, gastrointestinal tract, lungs,  muscular-skeletal system, and genital tract, often resembling manifestations of various autoimmune diseases. Predictive and diagnostic biomarkers of cGVHD are much needed to predict its onset, diagnose and monitor the disease, prognoses its course and outcome, and characterize its activity. At the University Hospital Center Zagreb a multidisciplinary team for cGVHD was formed in 2013 according to the team from the National Institutes of Health (NIH, Bethesda, USA).  This approach improved care of patients with cGVHD, but also provides interdisciplinary clinical and laboratory evaluation of this disease, collection of biologic samples with great potential for investigation of biomarkers of this complex and potentially lethal late complication after alloHSTC. The goal of this new 4-year project of the Croatian Science Foundation is to study possible new biomarkers of cGVHD (glycan analysis, coagulation factors, laboratory markers of inflammation, lymphocytes subpopulations, and monocytes) in a prospective longitudinal assessment of cGVHD patients, with their detail multidisciplinary evaluations using modern NIH standards for cGVHD.

Publikacije i ostale diseminacijske aktivnosti / Publications and other dissemination activities

Radovi u CC/SCI časopisima:
Papers published in CC/SCI Journals:

Peric Z, Skegro I, Durakovic N, Desnica L, Pulanic D, Serventi-Seiwerth R, Petricek I, Pavletic SZ, Vrhovac R. Amniotic membrane transplantation-a new approach to crossing the HLA barriers in the treatment of refractory ocular graft-versus-host disease. Bone Marrow Transplant. 2018 Nov;53(11):1466-1469. doi: 10.1038/s41409-018-0140-6.  Epub 2018 Aug 8.
Publikacije u drugim medicinskim časopisima:
Papers published in other medical journals:

1) Pulanić D, Desnica L, Serventi-Seiwerth R, Mravak-Stipetić M, Bilić E, Čeović R, Duraković N, Perić Z, Kraljević L, Bilić E, Bojanić I, Mazić S, Petriček I, Ljubas D, Vukić T, Klepac Pulanić T, Šepec BI, Barešić M, Karas I, Rajić Lj, Matić T, Dušek D, Grce M, Kurić L, Babić A, Giljević Z, Milošević M, Zadro R, Batinić D, Vrhovac R, Pavletić SZ, Nemet D. Daljnji razvoj multidisciplinarnog tima za kroničnu bolest presatka protiv primatelja KBC Zagreb – novi projekt. Bilten Krohema. 2017;9(1):36-38.
2) Pulanić D, Desnica L, Serventi-Seiwerth R, Mravak-Stipetić M, Bilić E,  Čeović R, Duraković N, Perić Z, Kraljević L, Bilić E, Bojanić I, Mazić S, Petriček I, Ljubas Kelečić D, Vukić T, Klepac Pulanić T, Šepec BI, Barešić M, Karas I, Rajić Lj, Matić T, Dušek D, Šaban N, Prenc E, Kurić L, Babić A, Ilić I, Rezo Vranješ V, Giljević Z, Milošević M, Pučić Baković M, Grce M, Lauc G, Zadro R, Batinić D, Vrhovac R, Pavletić SZ, Nemet D. Pet godina Multidisciplinarnog tima za kroničnu bolest presatka protiv primatelja KBC Zagreb. Bilten Krohema. 2018;10(1):38-41.
3)  Pulanić D, Desnica L, Perić Z, Samardžić A, Grce M, Vrhovac R, Pavletić SŽ. Zagreb – središte kronične bolesti presatka protiv primatelja početkom studenoga 2018. Bilten Krohema. 2018;10(2):41.
4) Novi HRZZ projekti na Medicinskom fakultetu. mef.hr 2018;37(1):94.
5) Poljak Ž.  Godišnja nagrada Zaklade KROHEM-a doc. Pulaniću i timu za kronični GVHD za izvrsnost i postignuća u hematologiji. Liječ Novine 2018;172:42-43.
6)  Nagrada KROHEM-a doc. dr. Draženu Pulaniću i timu za kronični GVHD. Medix 2018;132:46.
Poglavlja u knjigama:
Book chapters:

Dražen Pulanić. Organizacija multidisciplinarnog tima za kroničnu bolest presatka protiv primatelja (cGvHD) u KBC-u Zagreb – primjer dobre prakse. U: Opće zdravlje kroz oralno zdravlje – multidisciplinarni pristup. Marinka Mravak-Stipetić, Jadranka Sertić, Anka Jurišić Kvesić (urednice). Zagreb: Hrvatska komora dentalne medicine; ISBN 953951837-7 (u tisku). – sveučilišni priručnik.
Kongresni sažeci s međunarodnih sastanaka:
Congress abstracts from international meetings:

1) Peric Z, Skegro I, Durakovic N, Desnica L, Serventi-Seiwerth R, Pulanic D, Petricek I, Pavletic S, Vrhovac R. Severe ocular GVHD successfully treated with amniotic membrane transplantation – case series. ASH poster presentation 2017. Blood 2017;130:3270.
2) Farhadfar N, Desnica L, Pulanic D, Horn B. Therapy Decisions in Patients Evaluated in Specialized Chronic Graft-Versus-Host (cGVHD) Clinics Using 2014 Consensus Criteria for Diagnosis and Response Assessment. 2018 ASBMT BMT Tandem Meetings poster presentation. Biol Blood Marrow Transplant 2018; 24(3), Supplement: S187.
3) Babić A, Kurić L, Desnica L, Milošević M, Serventi Seiwerth R, Duraković N,  Perić Z, Mravak Stipetić M, Bilić E, Čeović R, Klepac Pulanić T, Petriček I, Vukić T, Šepec IB, Rajić Lj, Bilić E, Šaban N, Barešić M, Ljubas Kelečić D, Mazić S, Bojanić I, Zadro R, Vrhovac R, Pavletic SZ, Batinić D, Pulanić D. Subpopulation of monocytes and B regulatory cells in patient with chronic Graft-versus-Host disease. 23rd Congress of the European Hematology Association. EHA Learning Center. Pulanic D. Jun 16, 2018; 215778. Abstract: PS1492 HemaSphere. 2018;2 (S1): p 1–1113. doi: 10.1097/HS9.0000000000000060
4) Prenc E, Pulanic D, Ugrina I, Desnica L, Pucic Bakovic M, Stambuk J, Milosevic M, Serventi Seiwerth R, Durakovic N, Peric Z, Vrhovac R, Nemet D, Lauc D, Pavletic SZ. Discriminatory potential of IgG N-glycosylation in chronic graft-versus-disease. The 44th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant. 2018; 1–661.
5) Ljubas Kelecic D, Desnica L, Vukic T, Vranesic Bender D, Karas I, Barisic A, Domislovic V,  Peric Z, Durakovic N, Serventi Seiwerth R, Vrhovac R, Pulanic D, Nemet D, Pavletic SZ, Krznaric Z. Sarcopenia in cGVHD patients. ESPEN 2018 Abstract. Clinical Nutrition. 2018(September);37(Supplement 1):116.
6) Pulanic D, Samardzic A, Desnica L, Zadro R, Milošević M, Serventi Seiwerth R, Durakovic N, Peric Z, Coen Herak D, Milos M, Kraljevic L, Mravak Stipetic M, Bilic E, Ceovic R, Klepac Pulanic T, Petricek I, Saban N, Zelic KerepA, Ljubas Kelecic D, Vukic T,  Mazic S, Bojanic I, Bilić E, Batinic D, Vrhovac R, Pavletic SZ. High levels of FVIII and Von Willebrand Factor in chronic Graft-versus-Host Disease patients. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant 2019; in press.
7) Ljubas Kelecic D, Desnica L, Vukic T, Vranesic Bender D, Karas I, Barisic A, Horvat A, Sabol I, Samardzic A, Kraljevic L, Peric Z, Durakovic N, Serventi Seiwerth R, Vrhovac R, Pulanic D, Pavletic SZ, Krznaric Z. Factors affecting nutritional status in chronic GVHD. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant 2019; in press.
8) Desnica L, Hudolin T, Hauptman D, Serventi-Seiwerth R, Durakovic N, Peric Z, Vrhovac R, Pavletic SZ, Pulanic D. Buccal mucosa autograft and other treatment modalities for male genital chronic Graft-versus-Host disease – case series. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant 2019; in press.
9) Jurišić L, Samardžić A, Sabol A, Družinić V. Croatian experience with the Multdisciplinary team for cGVHD and long term complications after alloHSCT: a nurse`s perspective. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Nurses Poster Sessions-Information and Education. Bone Marrow Transplant 2019; in press.
10) Belev J, Samardžić A, Rendulić E, Družinić V. Nurse’s role in the Multidisciplinary team for cGVHD and long term complication after HSCT – a case report of a patient with multiple myeloma and multiple sclerosis. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Nurses Poster Sessions-Information and Education. Bone Marrow Transplant 2019; in press.
Kongresni sažeci s domaćih sastanaka:
Congress abstracts from domestic meetings:

1) Pulanić D. Biomarkeri kronične bolesti presatka protiv primatelja. Liječ Vjesn. 2017;139(Suppl.2):54-55. – oralna prezentacija rada  na 7. Hrvatskom kongresu hematologa s međunarodnim sudjelovanjem, Solaris, Hrvatska, 22.9.2017.
2) Barešić M, Čeović R, Vukić T, Desnica L, Vrhovac R, Pulanić D. Sličnosti i razlike sistemske skleroze i sklerotskog oblika kronične bolesti  presatka protiv primatelja. Liječ Vjesn. 2017;139(Suppl.2):58.
3) Omerbašić M, Desnica L, Vrhovac R, Duraković N, Perić Z, Pavletić Ž, Pulanić D, Dušek T. Funkcija štitnjače u bolesnika s kroničnom reakcijom transplantata protiv primatelja nakon alogenične transplantacije krvotvornih matičnih stanica. Liječ Vjesn. 2018;140(Suppl.2):51.
4) Brataljenović M, Pulanić D. Chronic Graft-versus-Host Disease: the importance of multidisciplinary approach. Book of Abstracts – Croatian Student Summit (CROSS). Liječ Vjesn. 2018;139(Suppl.1):49-50.
Diplomski radovi:
Graduate Thesis:

Matija Brataljenović. Kronična bolest presatka protiv primatelja kao najznačajnija kasna komplikacija nakon alogene transplantacije matičnih krvotvornih stanica. Sveučilište u Zagrebu Medicinski fakultet, 2018. godina, mentor doc. prim. dr. sc. Dražen Pulanić, dr. med.
Predavanja na stručnim i znanstvenim sastancima:
Lectures from professional and scientific meetings:

1) Dražen Pulanić: Novosti iz Tima za kronični GVHD KBC Zagreb (predavanje na sastanku Hrvatske kooperativne grupe za hematološke bolesti KROHEM, Vodice, 5. svibnja 2017.)
2) Dražen Pulanić: Organizacija multidisciplinarnog tima za kronični GvHD na KBC Zagreb – primjer dobre prakse  (predavanje na 1. Simpoziju o multidisciplinarnoj suradnji u dentalnoj medicini s međunarodnim sudjelovanjem „ Zajedništvom do oralnog i općeg zdravlja – multidisciplinarna suradnja u svakodnevnom kliničkom radu“, Zagreb, 27. travanj 2018.)
3) Dražen Pulanić: Need for multidisciplinary approach – 5 years of multidisciplinary cGVHD team in Zagreb (predavanje na 4th International Chronic GVHD Symposium and EBMT Transplant Complications Working Party Educational Course Joint Meeting, Zagreb, 9-10.11.2018

Radovi u CC/SCI časopisima:
Papers published in CC/SCI Journals:

Peric Z, Skegro I, Durakovic N, Desnica L, Pulanic D, Serventi-Seiwerth R, Petricek I, Pavletic SZ, Vrhovac R. Amniotic membrane transplantation-a new approach to crossing the HLA barriers in the treatment of refractory ocular graft-versus-host disease. Bone Marrow Transplant. 2018 Nov;53(11):1466-1469. doi: 10.1038/s41409-018-0140-6.  Epub 2018 Aug 8.
Publikacije u drugim medicinskim časopisima:
Papers published in other medical journals:

1) Pulanić D, Desnica L, Serventi-Seiwerth R, Mravak-Stipetić M, Bilić E, Čeović R, Duraković N, Perić Z, Kraljević L, Bilić E, Bojanić I, Mazić S, Petriček I, Ljubas D, Vukić T, Klepac Pulanić T, Šepec BI, Barešić M, Karas I, Rajić Lj, Matić T, Dušek D, Grce M, Kurić L, Babić A, Giljević Z, Milošević M, Zadro R, Batinić D, Vrhovac R, Pavletić SZ, Nemet D. Daljnji razvoj multidisciplinarnog tima za kroničnu bolest presatka protiv primatelja KBC Zagreb – novi projekt. Bilten Krohema. 2017;9(1):36-38.
2) Pulanić D, Desnica L, Serventi-Seiwerth R, Mravak-Stipetić M, Bilić E,  Čeović R, Duraković N, Perić Z, Kraljević L, Bilić E, Bojanić I, Mazić S, Petriček I, Ljubas Kelečić D, Vukić T, Klepac Pulanić T, Šepec BI, Barešić M, Karas I, Rajić Lj, Matić T, Dušek D, Šaban N, Prenc E, Kurić L, Babić A, Ilić I, Rezo Vranješ V, Giljević Z, Milošević M, Pučić Baković M, Grce M, Lauc G, Zadro R, Batinić D, Vrhovac R, Pavletić SZ, Nemet D. Pet godina Multidisciplinarnog tima za kroničnu bolest presatka protiv primatelja KBC Zagreb. Bilten Krohema. 2018;10(1):38-41.
3)  Pulanić D, Desnica L, Perić Z, Samardžić A, Grce M, Vrhovac R, Pavletić SŽ. Zagreb – središte kronične bolesti presatka protiv primatelja početkom studenoga 2018. Bilten Krohema. 2018;10(2):41.
4) Novi HRZZ projekti na Medicinskom fakultetu. mef.hr 2018;37(1):94.
5) Poljak Ž.  Godišnja nagrada Zaklade KROHEM-a doc. Pulaniću i timu za kronični GVHD za izvrsnost i postignuća u hematologiji. Liječ Novine 2018;172:42-43.
6)  Nagrada KROHEM-a doc. dr. Draženu Pulaniću i timu za kronični GVHD. Medix 2018;132:46.
Poglavlja u knjigama:
Book chapters:

Dražen Pulanić. Organizacija multidisciplinarnog tima za kroničnu bolest presatka protiv primatelja (cGvHD) u KBC-u Zagreb – primjer dobre prakse. U: Opće zdravlje kroz oralno zdravlje – multidisciplinarni pristup. Marinka Mravak-Stipetić, Jadranka Sertić, Anka Jurišić Kvesić (urednice). Zagreb: Hrvatska komora dentalne medicine; ISBN 953951837-7 (u tisku). – sveučilišni priručnik.
Kongresni sažeci s međunarodnih sastanaka:
Congress abstracts from international meetings:

1) Peric Z, Skegro I, Durakovic N, Desnica L, Serventi-Seiwerth R, Pulanic D, Petricek I, Pavletic S, Vrhovac R. Severe ocular GVHD successfully treated with amniotic membrane transplantation – case series. ASH poster presentation 2017. Blood 2017;130:3270.
2) Farhadfar N, Desnica L, Pulanic D, Horn B. Therapy Decisions in Patients Evaluated in Specialized Chronic Graft-Versus-Host (cGVHD) Clinics Using 2014 Consensus Criteria for Diagnosis and Response Assessment. 2018 ASBMT BMT Tandem Meetings poster presentation. Biol Blood Marrow Transplant 2018; 24(3), Supplement: S187.
3) Babić A, Kurić L, Desnica L, Milošević M, Serventi Seiwerth R, Duraković N,  Perić Z, Mravak Stipetić M, Bilić E, Čeović R, Klepac Pulanić T, Petriček I, Vukić T, Šepec IB, Rajić Lj, Bilić E, Šaban N, Barešić M, Ljubas Kelečić D, Mazić S, Bojanić I, Zadro R, Vrhovac R, Pavletic SZ, Batinić D, Pulanić D. Subpopulation of monocytes and B regulatory cells in patient with chronic Graft-versus-Host disease. 23rd Congress of the European Hematology Association. EHA Learning Center. Pulanic D. Jun 16, 2018; 215778. Abstract: PS1492 HemaSphere. 2018;2 (S1): p 1–1113. doi: 10.1097/HS9.0000000000000060
4) Prenc E, Pulanic D, Ugrina I, Desnica L, Pucic Bakovic M, Stambuk J, Milosevic M, Serventi Seiwerth R, Durakovic N, Peric Z, Vrhovac R, Nemet D, Lauc D, Pavletic SZ. Discriminatory potential of IgG N-glycosylation in chronic graft-versus-disease. The 44th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant. 2018; 1–661.
5) Ljubas Kelecic D, Desnica L, Vukic T, Vranesic Bender D, Karas I, Barisic A, Domislovic V,  Peric Z, Durakovic N, Serventi Seiwerth R, Vrhovac R, Pulanic D, Nemet D, Pavletic SZ, Krznaric Z. Sarcopenia in cGVHD patients. ESPEN 2018 Abstract. Clinical Nutrition. 2018(September);37(Supplement 1):116.
6) Pulanic D, Samardzic A, Desnica L, Zadro R, Milošević M, Serventi Seiwerth R, Durakovic N, Peric Z, Coen Herak D, Milos M, Kraljevic L, Mravak Stipetic M, Bilic E, Ceovic R, Klepac Pulanic T, Petricek I, Saban N, Zelic KerepA, Ljubas Kelecic D, Vukic T,  Mazic S, Bojanic I, Bilić E, Batinic D, Vrhovac R, Pavletic SZ. High levels of FVIII and Von Willebrand Factor in chronic Graft-versus-Host Disease patients. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant 2019; in press.
7) Ljubas Kelecic D, Desnica L, Vukic T, Vranesic Bender D, Karas I, Barisic A, Horvat A, Sabol I, Samardzic A, Kraljevic L, Peric Z, Durakovic N, Serventi Seiwerth R, Vrhovac R, Pulanic D, Pavletic SZ, Krznaric Z. Factors affecting nutritional status in chronic GVHD. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant 2019; in press.
8) Desnica L, Hudolin T, Hauptman D, Serventi-Seiwerth R, Durakovic N, Peric Z, Vrhovac R, Pavletic SZ, Pulanic D. Buccal mucosa autograft and other treatment modalities for male genital chronic Graft-versus-Host disease – case series. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Physicians Poster Sessions. Bone Marrow Transplant 2019; in press.
9) Jurišić L, Samardžić A, Sabol A, Družinić V. Croatian experience with the Multdisciplinary team for cGVHD and long term complications after alloHSCT: a nurse`s perspective. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Nurses Poster Sessions-Information and Education. Bone Marrow Transplant 2019; in press.
10) Belev J, Samardžić A, Rendulić E, Družinić V. Nurse’s role in the Multidisciplinary team for cGVHD and long term complication after HSCT – a case report of a patient with multiple myeloma and multiple sclerosis. The 45th Annual Meeting of the European Society for Blood and Marrow Transplantation: Nurses Poster Sessions-Information and Education. Bone Marrow Transplant 2019; in press.
Kongresni sažeci s domaćih sastanaka:
Congress abstracts from domestic meetings:

1) Pulanić D. Biomarkeri kronične bolesti presatka protiv primatelja. Liječ Vjesn. 2017;139(Suppl.2):54-55. – oralna prezentacija rada  na 7. Hrvatskom kongresu hematologa s međunarodnim sudjelovanjem, Solaris, Hrvatska, 22.9.2017.
2) Barešić M, Čeović R, Vukić T, Desnica L, Vrhovac R, Pulanić D. Sličnosti i razlike sistemske skleroze i sklerotskog oblika kronične bolesti  presatka protiv primatelja. Liječ Vjesn. 2017;139(Suppl.2):58.
3) Omerbašić M, Desnica L, Vrhovac R, Duraković N, Perić Z, Pavletić Ž, Pulanić D, Dušek T. Funkcija štitnjače u bolesnika s kroničnom reakcijom transplantata protiv primatelja nakon alogenične transplantacije krvotvornih matičnih stanica. Liječ Vjesn. 2018;140(Suppl.2):51.
4) Brataljenović M, Pulanić D. Chronic Graft-versus-Host Disease: the importance of multidisciplinary approach. Book of Abstracts – Croatian Student Summit (CROSS). Liječ Vjesn. 2018;139(Suppl.1):49-50.
Diplomski radovi:
Graduate Thesis:

Matija Brataljenović. Kronična bolest presatka protiv primatelja kao najznačajnija kasna komplikacija nakon alogene transplantacije matičnih krvotvornih stanica. Sveučilište u Zagrebu Medicinski fakultet, 2018. godina, mentor doc. prim. dr. sc. Dražen Pulanić, dr. med.
Predavanja na stručnim i znanstvenim sastancima:
Lectures from professional and scientific meetings:

1) Dražen Pulanić: Novosti iz Tima za kronični GVHD KBC Zagreb (predavanje na sastanku Hrvatske kooperativne grupe za hematološke bolesti KROHEM, Vodice, 5. svibnja 2017.)
2) Dražen Pulanić: Organizacija multidisciplinarnog tima za kronični GvHD na KBC Zagreb – primjer dobre prakse  (predavanje na 1. Simpoziju o multidisciplinarnoj suradnji u dentalnoj medicini s međunarodnim sudjelovanjem „ Zajedništvom do oralnog i općeg zdravlja – multidisciplinarna suradnja u svakodnevnom kliničkom radu“, Zagreb, 27. travanj 2018.)
3) Dražen Pulanić: Need for multidisciplinary approach – 5 years of multidisciplinary cGVHD team in Zagreb (predavanje na 4th International Chronic GVHD Symposium and EBMT Transplant Complications Working Party Educational Course Joint Meeting, Zagreb, 9-10.11.2018